We tested the Purely Green BioPesticide (Green Clean Product (GCP)) for its larvicidal activity on wild type larvae (Anopheles, Culex & Aedes spp.), collected from the field. GCP has shown its highest efficacy at concentration as low as 300 ppm (0.03%) for 100% larval mortality within 24-48 hrs in the laboratory experiments. It has shown lethal effect on pupal stage of mosquito development at the concentration of 300 ppm (0.03%) in 1.5 hrs. We tested GCP in small scale field trials where we found 0.05% is the minimum effective concentration for killing the larvae within 24-48 hrs in all possible way of application. GCP can be used for large-scale field trials.